|
KMID : 1007520220310111389
|
|
Food Science and Biotechnology
2022 Volume.31 No. 11 p.1389 ~ p.1399
|
|
|
Yellow pigment from gardenia fruit: structural identification and evaluation of cytotoxic activity in HepG2 cells by induction of apoptosis
|
|
Tang Liqin
Liu Haocheng
Fu Manqin
Xu Yujuan
Wen Jing
Wu Jijun
Yu Yuanshan
Lin Xian
Li Lu
Bu Zhibin
Yang Wanyuan
|
|
|
Abstract
|
|
|
|
The preparation process of yellow pigment (YP) from gardenia (Gardenia jasminoides) fruit was investigated, and the main components of YP were characterized by liquid chromatography-time of flight-mass spectrometer/mass spectrometer (LC-TOF?MS/MS). Furthermore, cytotoxic activity in HepG2 cells by induction of apoptosis was also evaluated. The preparation results indicated that the color value of YP was 498.34, which was 8.6 times higher than crude YP. Fifteen compounds in YP were identified, and crocins were the predominant compounds. The cell experiment results showed that YP inhibited the proliferation of HepG2 cells in a time- and dose-dependent manner. Moreover, YP also inhibited HepG2 cells in G2/M stage, increased the level of intracellular reactive oxygen species (ROS), and enhanced cell apoptosis. Real-time quantitative polymerase chain reaction (RT-PCR) analysis revealed the up-regulation of caspase-3, 8, 9, and bax and down-regulation of bcl-2 in HepG2 cells. Overall, these findings suggested that YP had potential cytotoxic activity in HepG2 cells by induction of apoptosis, which might be beneficial to human health.
|
|
|
KEYWORD
|
|
|
Yellow pigment, LC-TOF?MS/MS, HepG2, Crocins, Apoptosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|